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| Registros recuperados: 44 | |
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| Lemaire, Christophe; Versini, J; Bonhomme, Francois. |
| To investigate the origin and maintenance of the genetic discontinuity between Atlantic and Mediterranean populations of the common sea bass (Dicentrarchus labrax) we analysed the genetic variation at a fragment of mitochondrial cytochrome b sequence for 18 population samples. The result were also compared with new or previously published microsatellite data. Seven mitochondrial haplotypes and an average nucleotidic divergence of 0.02 between Atlantic and Mediterranean populations that matches a Pleistocene allopatric isolation were found. The frequency variation at the cytochrome b locus was many times greater between Atlantic and Mediterranean populations ((θ) over cap (C) = 0.67) than at microsatellite loci ((θ) over cap (N) = 0.02).... |
| Tipo: Text |
Palavras-chave: Reproductive isolation; Phylogeography; MtDNA; Microsatellites; Genetic transitions. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-776.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,... |
| Tipo: Text |
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf |
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| Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois. |
| Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using... |
| Tipo: Text |
Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf |
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| Desmarais, Erick; Belkhir, Khalid; Garza, John Carlos; Bonhomme, Francois. |
| Solitary LTR loci are the predominant form of LTR retrotransposons in most eukaryotic genomes. They originate from recombination between the two LTRs of an ancestral retrovirus and are therefore incapable of transposition. Despite this inactivity, they appear to have a substantial impact on the host genome. Here we use the murine RMER10 LTR family as an example to describe how such elements can reshape regions of the genome through multiple mutations on an evolutionary time scale. Specifically, we use phylogenetic analysis of multiple copies of RMER10 in rodent species, as well as comparisons of orthologous pairs in mouse and rat, to argue that insertions of members of this family have locally induced the emergence of tandem repeat loci as well as many... |
| Tipo: Text |
Palavras-chave: RMER10; Simple tandem repeats; LTR retrotransposons; Mouse genome. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2091.pdf |
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| Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice. |
| For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang & Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a... |
| Tipo: Text |
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| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf |
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| Fritsch, Manon; Morizur, Yvon; Lambert, E; Bonhomme, Francois; Guinand, B. |
| Sea bass (Dicentrarchus labrax) fisheries are becoming increasingly abundant along eastern Atlantic coastlines, especially in the ICES sub-areas IV, VII and VIII, but they are still not being managed by the European Union TAC (Total Allowed Catches) system. Scientific information about these sea bass stocks is limited, but the fishery is currently managed or modelled based upon these data, separated into the ICES divisions. Clearly, failure to manage satisfactorily the fishery may lead to a decline in stocks. We therefore investigated the structure of Atlantic sea bass populations at eight microsatellite loci in the Bay of Biscay and the English Channel and, additionally, at five selected loci in Ireland and Scotland. Genetic data showed no significant... |
| Tipo: Text |
Palavras-chave: Population structure; Microsatellite loci; Tagging; Mark recapture; Dicentrarchus labrax. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2401.pdf |
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| Hedgecock, Denis; Launey, Sophie; Pudovkin, A. I.; Naciri, Yamama; Lapegue, Sylvie; Bonhomme, Francois. |
| The great fecundity and very high larval mortality of most marine invertebrates and fish make possible substantial variance in the number of offspring contributed by adults to subsequent generations. The reproductive success of such organisms may thus resemble a sweepstakes lottery, in which a minority of progenitors succeeds in replacing an entire population, while the majority fails to procreate. One specific prediction of this hypothesis, that genetic diversity of newly settled cohorts should be less than that of the adult population, is tested in the present study. Microsatellite DNA markers were examined in naturally spawned juvenile European flat oysters Ostrea edulis (L.), collected over a 12-day period in 1993 from the western Mediterranean Sea,... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2616.pdf |
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| Rolland, Jean-luc; Bonhomme, Francois; Lagardere, Francoise; Hassan, M; Guinand, Bruno. |
| Spatial and temporal population genetic structures of the common sole, Solea solea, were studied in Northeastern Atlantic and Mediterranean Sea populations, using three polymorphic exon-primed intron-crossing (EPIC) markers. Results demonstrated significant multilocus differentiation among Eastern Mediterranean and a group composed by Western Mediterranean and Atlantic populations (theta = 0.150, P < 0.001), but also suggested unrecorded genetic differentiation of the Adriatic Sea population. No pattern of isolation-by-distance was recorded across the range covered by sampling, from the Kattegat to the Aegean Sea. Conversely to genetically structured Mediterranean populations, Atlantic populations ranging from Denmark to Portugal could be considered as... |
| Tipo: Text |
Palavras-chave: Marine fish; Genetic structure; Introns; Common sole. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2400.pdf |
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| Guinand, B; Rolland, Jean-luc; Bonhomme, Francois. |
| Coastal and estuarine areas in the Bay of Biscay are essential nursery habitats for sole (Solea solea). Using three intronic markers, we investigated patterns of genetic differentiation among cohorts of juveniles (0+, 1+, sub-adults) inhabiting four major nurseries in the Vilaine and Loire river estuaries, the Pertuis d' Antioche strait, and the Gironde estuary. Genetic differentiation was not significant for the age 0- and 1-groups, but was among sub-adult samples (theta(WC) = 0.017; P = 0.031). Hierarchical analyses of genotypic frequencies by nested multivariate ANOVA using redundancy analysis indicated that northern nurseries of Loire and Vilaine rivers had different patterns of genotypic variation with age than southern nurseries of the Pertuis d'... |
| Tipo: Text |
Palavras-chave: Selection; Genetic drift; EPIC PCR; Population genetics; Coastal nurseries; Juveniles; Flatfish. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4299.pdf |
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| Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre. |
| In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat... |
| Tipo: Text |
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf |
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| Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Camara, Mark D.; Lapegue, Sylvie. |
| Marine species with high fecundity and high early mortality may also have high variance in reproductive success among individuals due to stochastic factors, making successful reproduction a "sweepstakes." In some cases, the impact is sufficient to reduce the effective number of breeders in wild populations. We tested two predictions of the sweepstakes reproductive success hypothesis in a French Atlantic population of the European flat oyster, Ostrea edulis, by evaluating (1) whether individuals belonging to temporally discrete recruitment cohorts within a single reproductive season displayed reduced genetic variation relative to the entire adult population, and (2) whether these temporal cohorts of recruits were genetically differentiated from each other.... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7399.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| In order to document further the phenomena of variance in reproductive success in natural populations of the European flat oyster Ostrea Midis, two complementary studies based on natural and experimental populations were conducted. The first part of this work was focused on paternity analyses using a set of four microsatellite markers for larvae collected from 13 brooding females sampled in Quiberon Bay (Brittany, France). The number of individuals contributing as the male parent to each progeny assay was highly variable, ranging from 2 to more than 40. Moreover, paternal contributions showed a much skewed distribution, with some males contributing to 50-100% of the progeny assay. The second part of this work consisted of the analysis of six successive... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00012/12329/9222.pdf |
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| Cunha, Regina L.; Blanc, Francoise; Bonhomme, Francois; Arnaud-haond, Sophie. |
| Pearl oysters belonging to the genus Pinctada (Bivalvia: Pteriidae) are widely distributed between the Indo-Pacific and western Atlantic. The existence of both widely distributed and more restricted species makes this group a suitable model to study diversification patterns and prevailing modes of speciation. Phylogenies of eight out of the 11 currently recognised Pinctada species using mitochondrial (cox1) and nuclear (18S rRNA) data yielded two monophyletic groups that correspond to shell size and presence/absence of hinge teeth. Character trace of these morphological characters onto the molecular phylogeny revealed a strong correlation. Pinctada margaritifera appears polyphyletic with specimens from Mauritius grouping in a different clade from others of... |
| Tipo: Text |
Palavras-chave: Pinctada; Evolutionary patterns; Species complex; Allopatry; Biogeography. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00035/14635/14015.pdf |
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| Adjeroud, Mehdi; Guerecheau, Aurelie; Vidal-dupiol, Jeremie; Flot, Jean-francois; Arnaud-haond, Sophie; Bonhomme, Francois. |
| Clonality and genetic structure of the coral Pocillopora damicornis sensu lato were assessed using five microsatellites in 12 populations from four islands of the Society Archipelago (French Polynesia) sampled in June 2008. The 427 analysed specimens fell into 132 multilocus genotypes (MLGs), suggesting that asexual reproduction plays an important role in the maintenance of these populations. A haploweb analysis of ITS2 sequences of each MLG was consistent with all of them being conspecific. Genetic differentiation was detected both between and within islands, but when a single sample per MLG was included in the analyses, the populations turned out to be nearly panmictic. These observations provide further evidence of the marked variability in reproductive... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00171/28209/26518.pdf |
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| Gagnaire, Pierre-alexandre; Broquet, Thomas; Aurelle, Didier; Viard, Frederique; Souissi, Ahmed; Bonhomme, Francois; Arnaud-haond, Sophie; Bierne, Nicolas. |
| Estimating the rate of exchange of individuals among populations is a central concern to evolutionary ecology and its applications to conservation and management. For instance, the efficiency of protected areas in sustaining locally endangered populations and ecosystems depends on reserve network connectivity. The population genetics theory offers a powerful framework for estimating dispersal distances and migration rates from molecular data. In the marine realm, however, decades of molecular studies have met limited success in inferring genetic connectivity, due to the frequent lack of spatial genetic structure in species exhibiting high fecundity and dispersal capabilities. This is especially true within biogeographic regions bounded by well-known... |
| Tipo: Text |
Palavras-chave: Connectivity; Gene flow; Marine conservation; Population genomics; Population structure. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38592/37120.pdf |
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| Registros recuperados: 44 | |
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